Figure 7.

Myc knockdown attenuates IL-1β-induced apoptosis in INS-1 cells. INS-1 cells were transfected with siRNA directed against Myc, a scrambled siRNA control (NS) or vehicle (INS-1) for 16 h. Thereafter, cells were exposed to 150 pg/mL IL-1β (closed bars) or vehicle (open bars) for 24 h. (a) Total Myc protein levels and (b) cleaved caspase 3 were analyzed by immunoblotting. Actin was used as the loading control. Data are shown as means ± SEM of 3-4 independent experiments. Blots shown are representative. (c) Apoptosis was measured as relative levels of cytoplasmic nucleosomes in lysates measured by the Roche Cell Death Elisa kit. Data are shown as means ± SEM of 3-4 independent experiments. ^∗ P < 0.05 and ^∗∗ P < 0.01 versus IL-1β exposed NS.