Table 3.
Comparative partial proteomic analysis of rpoN2 mutant compared to wild type of B. pseudomallei. The spot numbers assigned in this study are identical to the number of spots in B. pseudomallei 2DE reference map [18].
| Protein orthologues | Expression alteration ratio in B. pseudomallei strains | pI | MW | Spot number | |
|---|---|---|---|---|---|
| Wild type | rpoN2 mutant | ||||
| (1) Cell envelope biogenesis and outer membrane | |||||
| Acetyltransferase (GNAT) family protein | 1 | ↑ 8.30 | 4.9 | 50.17 | 9 |
| UDP-N-acetylmuramyl pentapeptide synthase | 1 | ↑ 5.02 | 5.7 | 41.55 | 2 |
| (2) Energy metabolism | |||||
| Polyphosphate kinase 2 family (Ppk2) | 1 | ↑ 3.17 | 4.89 | 44.26 | 40 |
| ATB synthase subunit B | 1 | ↑ 3.40 | 4.97 | 53.93 | 15 |
| NADH-dehydrogenase delta subunit | 1 | 0.17 ↓ | 5.34 | 36.59 | 26 |
| Aldehyde dehydrogenase (NAD) family protein | 1 | 0.19 ↓ | 6.02 | 55.39 | 24 |
| (3) Carbohydrate metabolism | |||||
| Glyceraldehyde 3-phosphate dehydrogenase (GapA) | 1 | ↑ 3.38 | 4.82 | 20.88 | 48 |
| Nucleoside-diphosphate-sugar epimerases | 1 | ↑ 12.58 | 5.21 | 27.78 | 56 |
| (4) Lipid metabolism | |||||
| 4-Hydroxyl-3-methylbut-2-enyl diphosphate reductase (lspH) | 1 | ↑ 5.07 | 5.94 | 40.81 | 71 |
| Nonribosomally encoded peptide/polyketide synthase (CmaB) | 1 | 0.21 ↓ | 6.19 | 37.19 | 73 |
| (5) Amino acid metabolism | |||||
| Phosphoribosyl formimino-5-aminoimidazole carboxamide ribotide isomerase (HisA) | 1 | 0.20 ↓ | 4.42 | 31 | 83 |
| Cysteine synthase (CysM) | 1 | 0.33 ↓ | 5.75 | 36.11 | 80 |
| (6) Nucleotide metabolism | |||||
| Orotidine 5′-phosphate decarboxylase | 1 | ↑ 6.42 | 4.99 | 32.79 | 88 |
| (7) Translation | |||||
| 50S ribosomal protein L3 | 1 | ↑ 3.49 | 4.14 | 27.8 | 150 |
| (8) Transcription | |||||
| Transcriptional regulator, ArsR family 1 | 1 | 0.20 ↓ | 4.92 | 37.92 | 124 |
| (9) Posttranslational modification | |||||
| Thioredoxin | 1 | ↑ 3.37 | 5.97 | 16.15 | 179 |
| Peptidyl-prolyl cis-trans isomerase B | 1 | ↑ 22.15 | 6.05 | 19.99 | 174 |
| Glutathione S-transferase | 1 | ↑ 3.05 | 6.5 | 22 | 176 |
| (10) Cell motility, intracellular trafficking, and secretion | |||||
| Type III effector protein BipD | 1 | ↑ 5.04 | 4.38 | 34.37 | 194 |
| Type III secretion chaperone LcrH/SycD | 1 | ↑ 6.23 | 4.92 | 18.52 | 190 |
| (11) Stress responses | |||||
| Carbohydrate porin, OprB family | 1 | ↑ 3.16 | 5.01 | 38.71 | 233 |
| (12) Hypothetical protein | |||||
| Hypothetical protein BPSL0349 | 1 | ↑ 3.15 | 5.28 | 16.53 | 253 |
| Hypothetical protein BPSS0931 | 1 | 0.03 ↓ | 4.64 | 23.63 | 274 |