Table 2.
Composition of the solutions used to prepare the simulated gastrointestinal solutions and amounts required to prepare the gastric secretion (GS) and duodenum secretion (DS) media.
| Saliva | Basal gastric secretions | Active gastric secretions | Basal pancreatic secretions | Lipase | Active pancreatic secretions | Bile | |
|---|---|---|---|---|---|---|---|
| Composition | 6 mM KCl | 20 mM HCl | 15 mM KCl | 100 mM NaCl | 0.9 mM HCl | 20 mM NaCl | 100 g/L Oxgall |
| 62 mM NaHCO3 |
10 mM KCl | 5 mM NaCl | 50 mM NaHCO3 |
10 mM KCl | 140 mM NaHCO3 |
||
| 15 mM NaCl | 90 mM NaCl | 150 mM HCL |
10 mM KCl | 90 mM NaCl | 10 mM KCl | ||
| 6 mM K2HPO4·3H2O |
100 U/mL Pepsin |
1000 U/mL Pepsin |
1.2 g/L pancreatin |
300 U/mL lipases | 12 g/L pancreatin |
||
| 0.0066 mM CaCl2·2H2O |
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| 2.2 g/L Mucin | |||||||
| 2000 U/mL α-amylase |
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|
| |||||||
| Amount of GS medium (mL) | 39 | 16 | 35 | 11 | |||
|
| |||||||
| Amount of DS medium (mL) | 17 | 7 | 15 | 3 | 5 | 37 | 17 |