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. 2014 Jun 12;2014:749630. doi: 10.1155/2014/749630

Table 2.

Composition of the solutions used to prepare the simulated gastrointestinal solutions and amounts required to prepare the gastric secretion (GS) and duodenum secretion (DS) media.

Saliva Basal gastric secretions Active gastric secretions Basal pancreatic secretions Lipase Active pancreatic secretions Bile
Composition 6 mM KCl 20 mM HCl 15 mM KCl 100 mM NaCl 0.9 mM HCl 20 mM NaCl 100 g/L
Oxgall
62 mM 
NaHCO3
10 mM KCl 5 mM NaCl 50 mM 
NaHCO3
10 mM KCl 140 mM 
NaHCO3
15 mM NaCl 90 mM NaCl 150 mM
 HCL
10 mM KCl 90 mM NaCl 10 mM KCl
6 mM 
K2HPO4·3H2O
100 U/mL
Pepsin
1000 U/mL
Pepsin
1.2 g/L
pancreatin
300 U/mL lipases 12 g/L
pancreatin
0.0066 mM 
CaCl2·2H2O
2.2 g/L Mucin
2000 U/mL 
α-amylase

Amount of GS medium (mL) 39 16 35 11

Amount of DS medium (mL) 17 7 15 3 5 37 17