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government organization in the United States.
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information only on official, secure websites.
Ranges of variables used in CCD.
| S. no | Variables | Code | Levels | ||||
|---|---|---|---|---|---|---|---|
| −2.37 | −1 | 0 | 1 | 2.37 | |||
| 1 | Temperature (°C) | A | 20 | 25 | 30 | 35 | 40 |
| 2 | Substrate concentration (g/L) | B | 1 | 2 | 3 | 4 | 5 |
| 3 | pH | C | 6 | 6.5 | 7 | 7.5 | 8 |
| 4 | Agitation speed (rpm) | D | 50 | 100 | 150 | 200 | 250 |
| 5 | Inoculum size (mL) | E | 1 | 2 | 3 | 4 | 5 |
