. 2016 Jan 20;2016:5854285. doi: 10.1155/2016/5854285

Table 1.

Primers used for amplification of S. Typhi and S. Paratyphi A serotypes in clinical specimens.

Primer sequence	Target gene	Salmonella serotype	Amplicon size (bp)	PCR condition (number of cycles)	References
ST1 5′-ACTGCTAAAACCACTACT-3′ ST2 5′-TTAACGCAGTAAAGAGAG-3′	fliC	Typhi	495 bp	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35)	Song et al. [17]
ST3 5′-AGATGGTACTGGCGTTGCTC-3′ ST4 5′-TGGAGACTTCGGTCGCGTAG-3′	fliC	Typhi	364 bp	94°C, 1 min; 63°C, 1 min; 72°C, 1 min (35)	Frankel [18]

staA F1: 5′-TGGTTACATGACCGGTAGTC-3′ staA R1: 5′-TAGCTGCCGCAATGGTTATG-3′	staA	Typhi	537 bp	94°C, 1 min; 57°C, 1 min; 72°C, 1 min (35)	Pratap et al. [19]
staA F2: 5′-CATCGGCACGAACGTAAGAC-3′ staA R2: 5′-TCAAGCGACTGATGGTGACG-3′	staA	Typhi	377 bp	94°C, 1 min; 65°C, 1 min; 72°C, 1 min (35)	Pratap et al. [19]

stkG F1: 5′-CGTTTACTGAGGTCACAGGCATC-3′ stkG R1: 5′-CACATTGTTCTCGGAGACCCCA-3′	stkG	Paratyphi A	427 bp	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35)	Pratap et al. [20]
stkG F2: 5′-CAATGGCTTCTGGCGAACTGTC-3′ stkG R2: 5′-TGGAGAAAGATCAGACCACCGAG-3′	stkG	Paratyphi A	229 bp	94°C, 1 min; 61°C, 1 min; 72°C, 1 min (35)	Pratap et al. [20]