Table 3.
Mitochondrial parameters.
| Normoxia | Hypoxia | Reoxygenation | |
|---|---|---|---|
| MDA: nmol × mg−1 mitochondrial protein (n = 6; mean ± SD; N versus R and R versus R0.25 p < 0.05) | |||
| 17.85 ± 2.79 (100%) | 21.24 ± 3.53 (119.0%) | 29.52 ± 9.47 (165.4%; N versus R; p < 0.05) | |
| Curc. 0.25 µM | 17.68 ± 4.40 (99.1%) | 16.47 ± 1.99 (92.3%; R versus R0.25; p < 0.05) | |
| Curc. 0.5 µM | 19.93 ± 3.62 (111.7%) | 24.45 ± 3.24 (137.0%) | |
|
| |||
| GSH: nmol × mg−1 protein (n = 6 in each group; mean ± SD) | |||
| 40.6 ± 10.9 (100%) | 22.9 ± 10.7 (56.4%) | 14.3 ± 4.3 (35.2%) | |
| N versus H (p = 0.011) | N versus R (p < 0.001) | ||
| Curc. 0.25 µM | 19.5 ± 7.1 (48.0%) | 27.0 ± 11.2 (66.5%) | |
| R versus R0.25 (p < 0.05) | |||
| Curc. 0.5 µM | 23.2 ± 7.0 (57.1%) | 28.3 ± 8.0 (69.7%) | |
| R versus R0.5 (p < 0.05) | |||
|
| |||
| GPx: nmol × min−1 × mg−1 protein (n = 6 in each group; mean ± SD) | |||
| 324.1 ± 115.0 (100%) | 392.2 ± 150.1 (121.1%) | 826.1 ± 268.0 (255.2%) | |
| H versus R (p < 0.05) | N versus R (p < 0.05) | ||
| Curc. 0.25 µM | 513.3 ± 59.4 (158.6%) | 498.6 ± 44.5 (153.8%) | |
| R versus R0.25 (p < 0.05) | |||
| Curc. 0.5 µM | 664.4 ± 206.3 (205.3%) | 665.0 ± 219.1 (205.7%) | |
|
| |||
| GR: nmol × min−1 × mg−1 protein (n = 6 in each group; mean ± SD) | |||
| 77.2 ± 15.7 (100%) | 45.3 ± 17.1 (58.8%) | 22.2 ± 9.6 (29.3%; N versus R p < 0.05) | |
| Curc. 0.25 µM | 36.9 ± 16.9 (48.8%) | 35.4 ± 16.0 (46.8%) | |
| Curc. 0.5 µM | 47.3 ± 18.6 (62.5%) | 46.9 ± 18.5 (62.0%; R versus R0.5 p = 0.006) | |
MDA, malondialdehyde; GSH, reduced glutathione; GPx, glutathione peroxidase; GR, glutathione reductase; N, normoxia; H, hypoxia; R, reoxygenation; R0.25, reoxygenation with 0.25 µM curcumin (curc.); R0.5, reoxygenation with 0.5 µM curc.