Figure 4.

Effects of CPX and DEF on HeLa cell protein synthesis and accumulation. A, extracts of HeLa cells treated with CPX or DEF and control cells were resolved in 2D gels and stained with SYPRO Ruby. Sections show representative differentially expressed stained spots (arrows). B, as in A, except that the cells were labeled with [³⁵S]-methionine and -cysteine and spots were detected by autoradiography. C, enumeration of 2D gel spots that changed after treatment with CPX (left numbers) or DEF (right numbers) as detected by staining (columns) and labeling (rows). Bold-bordered numerals denote concordant spots, which were examined further. Italicized numerals denote discordant spots.