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. Author manuscript; available in PMC: 2016 Feb 8.
Published in final edited form as: Cancer Res. 2013 Nov 12;74(2):552–562. doi: 10.1158/0008-5472.CAN-13-0474

Figure 5.

Figure 5

Identification and expression of drug-regulated proteins. A, identity and properties of proteins affected by both CPX and DEF and identified by mass spectrometry. Molecular weights (MW) and isoelectric points (pI) were calculated from protein sequences (www.phosphositeplus.org and www.expasy.org). B–G, gene expression analysis of drug effects in HeLa cells. Top, immunoblot analysis of the downregulated (B–E) and upregulated (F and G) proteins with actin as a control. Bar graphs, protein levels relative to actin were evaluated by immunoblotting (filled bars) and corresponding RNA levels were assayed using RT-PCR (gray bars). Data are means of 3 independent experiments with SD.