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Depletion of mature eIF5A induces IκB and inhibits transcription directed by the HIV-1 promoter. HeLa cells were transfected with siRNAs directed against eIF5A, DHS, or DOHH or control siRNA, then with pCMV-Renilla and the HIV-1 molecular clone pNL4-3lucE− encoding firefly luciferase (FF). A, immunoblot analysis. eIF5A was detected with BD antibody. B, expression of FF luciferase normalized to Renilla luciferase activity with SD.
