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. 2015 Sep 21;6(30):28621–28632. doi: 10.18632/oncotarget.5765

Figure 6. Addition of CLEC-2 protein/podoplanin antibody to T cells induces T regulatory cells.

Figure 6

A. Histograms depict the expression of Podoplanin of on naïve, central memory and effector memory CD4 T cells. Figure is representative of 3 such experiments. B. Histogram depicts the binding of CLEC-2 protein on freshly isolated CD4 T cells. Figure is representative of 3 such experiments. Purified T cells were cultured with CLEC-2 protein for 96h. C. Bar graph depicts the percentage of FoxP3+ cells in gated CD4+CD25+CD127loFoxP3+ cells in CD4+T cells cultured with CLEC-2. D. Bar graphs depict the production of TGF-β, IL-10 and IFN-γ by T cells cultured in the presence of CLEC-2 protein. Data is mean +/− S.E. of 4 different subjects. Purified T cells were cultured with either podoplanin antibod (podo) or podo+2nd antibody or isotype antibody (iso) + 2nd antibody for 96h. C. Bar graph depicts the percentage of FoxP3+ cells in gated CD4+CD25+CD127loFoxP3+ cells. D. Bar graphs depict the production of TGF-β, IL-10 and IFN-γ by T cells. Data is mean +/− S.E. of 4 different subjects.