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. 2015 Aug 17;6(30):28882–28894. doi: 10.18632/oncotarget.4921

Figure 2. MiR-1207 overexpression promotes cancer stem–like traits in ovarian cancer.

Figure 2

A–B. Representative micrographs (A) and quantification (B) of tumor sphere formation in miR-1207 overexpression or vector cells. Scale bar, 100 μm. C–D. Hoechst 33342 dye exclusion assay showing the effect of miR-1207 overexpression cells or vector cells on the distribution of SP+ (C) and CD133+ cells (D) determined using flow cytometric analysis. E. Real-time PCR analysis of mRNA expression of pluripotency-associated markers in the cells. Transcript levels were normalized to GAPDH expression. Error bars represent the mean ± SD of three independent experiments. *P < 0.05.