Figure 2. Stat5-deficient HSCs have reduced expression of HSC/quiescence associated genes and increased expression of lymphoid-lineage genes.

Bone marrow cells from either Vav1-Cre+/Stat5ab^fl/fl and littermates or wild-type and Stat5ab^null/null fetal liver transplanted chimeric mice were collected and the KLS fractions were isolated by flow cytometry sorting. Real-time PCR analysis of purified KLS from wild-type or Stat5ab^null/null fetal liver transplanted mice (marked as solid diamonds) or KLS from Stat5ab^fl/fl and Vav1-Cre+/Stat5ab^fl/fl mice (open triangles) is presented as fold changes of individual gene expression between Stat5ab^null/null and wild-type KLS cells after the normalization to GAPDH using the ΔΔCt method. The primer sets analyzed are shown on the top with the decreased transcripts shown to the left and the increased transcripts shown to the right. The middle primer sets were not significantly changed.