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. 2015 Aug 21;6(30):28999–29015. doi: 10.18632/oncotarget.4905

Figure 2. ADAR1-mediated regulation of melanoma immune resistance is dependent on cell-cell interaction.

Figure 2

A. TIL52 were pre-incubated with conditioned media (CM) from 624mel ADAR1-KD or Scramble cells. After 1 h, the cells were co-incubated overnight with non-manipulated 624mel cells (E:T – 15:1). Specific lysis of 624mel cells was assessed by flow cytometry. B. 624mel ADAR1-KD or Scramble cells were loaded in the upper well of a transwell chamber. Non-manipulated 624mel cells were co-incubated overnight with TIL14 (E:T – 15:1) in the transwell lower well. Specific lysis of 624mel cells was assessed by flow cytometry. C. ADAR1-KD (dotted line) and Scramble (black line) cells were stained with anti-HLA Class I antibody (W6/32). Expression was analyzed by flow cytometry. D. ADAR1-KD (dotted line) and Scramble (black line) cells were stained with anti-melanoma antibody gp100/MART1). Expression was analyzed by flow cytometry. Experiments were performed three times in triplicates. Flow cytometry figures show one representative experiment.