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. 2015 Aug 21;6(30):28999–29015. doi: 10.18632/oncotarget.4905

Figure 3. ADAR1 regulates ICAM1 expression, which contributes to TIL mediated killing.

Figure 3

A. ICAM1 protein expression in ADAR1-p110 (dotted line) and Mock cells (black line) was analyzed by extracellular flow cytometry staining using ICAM1 monoclonal antibody. B. LFA-1 protein expression in TIL52, TIL14 and JKF6 was analyzed by extracellular flow cytometry staining using human LFA-1 alpha antibody. C. ADAR1-p110 and Mock cells were incubated with different concentrations of IgG1 control (isotype) or ICAM1 antibody. After 1 h, cells were co-incubated with JKF6 for 9 h (E:T - 15:1). Specific lysis of melanoma cells was assessed by LDH release. Experiments were performed three times in triplicates. Flow cytometry figures show one representative experiment.