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. 2015 Aug 24;6(30):29016–29033. doi: 10.18632/oncotarget.5032

Figure 5. PAI-1 knockdown in the SCC9 cell line decreases migration, enhances proliferation and promotes apoptotic induction.

Figure 5

A. Expression of SERPINE1 in SCC9 and cells transduced with shRNAi (SCC9 shRNA004 and SCC9 shRNA159, as assessed by Western-Blot (above) or RT-PCR (bellow); B. Representative images showing changes in cell morphology after the inhibition of SERPINE1 expression; C. Transwell migration and D. proliferation assays in SCC9, SCC9 shRNA004 and SCC9 shRNA159 cells; E. Representative images of DAPI stained nuclei before and after 16 hours of 40 μM cisplatin treatment in SCC9 and shRNA transduced cells, showing higher apoptosis in PAI-1 downregulated cells; F. Western blot analysis of P-Akt, PI3K and ERK in SCC9, SCC9 shRNA004, UM-SCC-74B and 74BSerp1up cells; G. western blot analysis of P-Akt in cells treated during 48 hours with the AKT inhibitor MK-2206; H. Transwell migration assays in cells treated with the MK-2206 inhibitor; I. SERPINE1 and P-Akt protein expression in cells treated 48 hours with the SERPINE1 inhibitor PAI-039. J. Combination of cisplatin and AKT inhibitor MK2206 in UM-SCC-74B and 74BSerpE1up cells.*p < 0.05