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. 2015 Aug 5;6(30):29048–29059. doi: 10.18632/oncotarget.4804

Figure 3. TAZ is a target gene of miRNA-338-3p.

Figure 3

A, B. HCC cell lines were transfected with miRNA-338-3p mimics and mimics control (A) or miRNA338-3p inhibitor and its corresponding negative control (B) The protein level of TAZ was determined by western blot. β-actin served as the internal control. C. Schematic of the reporter plasmids containing wild or mutant 3′UTR of TAZ. The complementary site of the seed sequence of miRNA-338-3p was selected for mutation. D, E. HCC cell lines were transfected with miRNA-338-3p mimics and its negative control (D) or miRNA-338-3p inhibitor and its negative control (E) Luciferase activity was detected 48 h after transfection. Renilla activity served as transfection control. The experiments were repeated for 3 times and data was shown as mean ± SD. *p < 0.05, **p < 0.01.