Figure 3. miR-155 overexpression inhibits invasion of metastatic CL16 cancer cells in vitro, but has no effect on proliferation or apoptosis.

A. Immunohistochemical staining of lung tumors derived from i.v. injected CL16-miR-155 or CL16-Ctrl cells for the apoptotic marker cleaved Caspase-3 showed very few apoptotic cells and no difference between the two groups. B. Staining of the same lung tumors for the proliferation marker Ki-67 showed similar frequency of Ki-67-positive tumors cells in the two groups. C. CL16-miR-155 cells showed significantly decreased invasion capability compared to CL16-Ctrl (student t-test, p = 0.028) when evaluated in an in vitro invasion assay. A representative experiment out of 3 is shown. D. In the same experiment, no difference in proliferation in vitro was observed between the CL16-miR-155 and CL16-Ctrl cells.