Figure 4. No difference in morphology, E-cadherin or vimentin expression was observed between CL16-miR-155 and CL16-Ctrl cells.
A. Phase-contrast images showing that both CL16-miR-155 and CL16-Ctrl cells exhibit mesenchymal-like morphology in vitro. Objective 10x, numerical aperture NA 0.3. Scale bar 100 μm. B. Staining for E-cadherin and vimentin in metastasis from i.v. injection model and cells grown in vitro as determined by immunohistochemistry. Objective 100x, numerical aperture NA 1.25 Scale bar 20 μm.