Table 2. Significant differences in levels of factors in wound fluid in relation to NAC.

Factor	Group	N	M pg/ml	X25% pg/ml	X75% pg/ml	P value
IL-1β	Non-NAC	10	397.12	29.28	584.31	0.011
	NAC	7	1093.47	814.88	3555.27
IL-4	Non-NAC	10	16.55	12.83	19.71	0.024
	NAC	7	21.15	18.46	28.27
IL-6	Non-NAC	10	118763.50	75960.74	216038.77	0.015
	NAC	7	240568.27	213409.31	302916.58
IL-17F	Non-NAC	10	71.21	45.92	86.97	0.002
	NAC	7	101.69	91.78	128.01
IL-21	Non-NAC	10	604.90	349.05	855.75	0.008
	NAC	7	1113.52	875.29	1622.89
IL-23	Non-NAC	9	43.63	17.61	73.48	0.003
	NAC	7	100.69	94.86	155.20
IL-25	Non-NAC	10	11.59	5.06	14.00	0.010
	NAC	7	16.71	13.82	27.06
IL-31	Non-NAC	10	43.34	22.60	51.08	0.036
	NAC	7	82.66	55.78	107.33
IFNγ	Non-NAC	8	145.83	85.98	197.05	0.049
	NAC	7	212.37	158.29	335.37
CD40L	Non-NAC	10	301.67	141.66	339.24	0.001
	NAC	7	602.84	486.55	808.91
TNFα	Non-NAC	10	101.82	41.48	169.05	0.025
	NAC	7	223.80	149.13	232.31
CXCL1	Non-NAC	23	834.00	394.76	2816.39	0.027
	NAC	8	2762.10	1920.99	5550.09
CXCL2	Non-NAC	23	227.14	143.20	917.18	0.011
	NAC	8	1261.87	546.68	4006.32
CXCL5	Non-NAC	23	3602.10	2132.47	17961.81	0.017
	NAC	8	32123.87	13323.82	52560.84
CCL3	Non-NAC	23	173.66	53.85	515.78	0.047
	NAC	8	926.39	175.88	1051.18
CCL7	Non-NAC	23	882.29	623.90	1111.82	0.030
	NAC	8	1574.15	923.87	2448.91
CCL20	Non-NAC	23	3061.31	1526.12	5531.57	0.030
	NAC	8	7083.39	5023.03	9759.27

Levels are expressed as median and range in X_25%, X_75%.

Differences were considered to be significant at the P < 0.05 level. Some data was missing because the concentration was undetectable.

IL = Interleukin; IFNγ = Interferon γ; CD40L = CD40 ligand; NAC = neoadjuvant chemotherapy; Non-NAC = never received neoadjuvant chemotherapy.

Cytokines and chemokines detection assays were two independent tests.