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A. ANO9 expression in 6 CRC cell lines was detected by RT-PCR. GAPDH was amplified as an internal control. B. In recurrent CRC, ANO9 mRNA levels were significantly decreased compared to the CRC without recurrence (Wilcoxon Signed Ranks Test, P < 0.05). C. Western blots analysis of ANO9 protein expression in CRC tissues. R indicates recurrence samples; F, samples free of recurrence.
