Skip to main content
. 2015 Aug 8;6(30):29396–29412. doi: 10.18632/oncotarget.5120

Figure 1. Identification of phosphorylation of S165 on YBX1.

Figure 1

A. SDS-PAGE gel stained with GelCode blue, showing a strong Flag-tagged YBX1 band that was pulled down using anti-Flag antibody via co-immunoprecipitation. B. Mass Spectrometry data for YBX1, showing that in response to IL-1β treatment, serine 165 (S165) is phosphorylated. A mass shift of 80Da was observed, indicating the existence of phosphorylation modification. C. Western blot, showing that S102 is not phosphorylated in 293 cells upon IL-1β stimulation. In contrast, S102 is phosphorylated in breast cancer MCF7 cells upon IGF-1 treatment.