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. 2015 Aug 8;6(30):29396–29412. doi: 10.18632/oncotarget.5120

Figure 3. Phosphorylation of S165 is critical for the expression of a subgroup of NF-κB target genes.

Figure 3

A. Comparative analysis of YBX1-S165A mutant with WT-YBX1 overexpressing cells on YBX-inducible NF-κB target genes. B. A short list of typical NF-κB inducible genes that can be up regulated by WT-YBX1 but not by YBX1-S165A. C. Quantitative PCR (qPCR) analysis, showing confirmation of microarray data. Three genes: OSM, PFKB2, and TRIM14 show reduced gene expression in the mutant S165A-YBX1 as compared to that of WT-YBX1. The data represent the means ± SD from three independent experiments. *P < 0.05 vs. Ctrl group; #P < 0.05 vs. WT-YBX1 group. D. qPCR analysis, showing that PFKB2, OSM and TRIM14 genes were strongly induced upon IL-1β treatment. This effect was further augmented by the overexpression of WT-YBX1. However, the expression of these genes were greatly decreased upon shRNA knockdown of YBX1. The data represent the means ± SD for three independent experiments. *P < 0.01 vs. Ctrl group; #P < 0.01 vs. WT-YBX1 group; $P < 0.05 vs. WT-YBX1 group, with IL-1β treatment.