Table 3. siRNA-induced downregulation of mRNA measured by RT-qPCR.
| Cell line | Quantified mRNA | Targeted siRNA | siRNA GFP | Inhibition (%) | p-value (ANOVA) | ||
|---|---|---|---|---|---|---|---|
| mean (fMol) | sem | mean (fMol) | sem | ||||
| GHD | ercc1 | 8.6E-06 | 2.0E-06 | 2.1E-05 | 5.0E-06 | 58.6 | 0.019 |
| ercc2 | 1.7E-07 | 3.9E-08 | 4.3E-07 | 7.1E-08 | 61.6 | 0.011 | |
| mutyh | 5.0E-05 | 1.3E-05 | 1.7E-04 | 3.6E-05 | 71.0 | 0.004 | |
| pnkp | 8.0E-06 | 1.6E-06 | 5.0E-05 | 1.6E-05 | 83.8 | 0.005 | |
| U373 | ercc1 | 1.9E-06 | 8.2E-07 | 8.4E-06 | 2.3E-06 | 77.4 | 0.018 |
| ercc2 | 1.2E-07 | 3.0E-08 | 2.8E-06 | 7.9E-07 | 95.7 | 0.003 | |
| mutyh | 2.6E-05 | 7.6E-06 | 9.5E-05 | 1.8E-05 | 72.9 | 0.007 | |
| pnkp | 3.6E-05 | 9.6E-06 | 6.5E-05 | 1.6E-05 | 44.2 | 0.013 | |
| mgmt | 4.2E-06 | 5.2E-07 | 2.1E-05 | 1.0E-06 | 80.4 | 0.001 | |
All used siRNAs significantly reduced expression of their respective targets, when compared to the control siRNA (GFP). Measures were performed twice from each cell sample and results were expressed as the mean of at least three independent samples (originating from independent in vitro experiments)