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. 2015 Aug 20;6(30):29456–29468. doi: 10.18632/oncotarget.4909

Table 3. siRNA-induced downregulation of mRNA measured by RT-qPCR.

Cell line Quantified mRNA Targeted siRNA siRNA GFP Inhibition (%) p-value (ANOVA)
mean (fMol) sem mean (fMol) sem
GHD ercc1 8.6E-06 2.0E-06 2.1E-05 5.0E-06 58.6 0.019
ercc2 1.7E-07 3.9E-08 4.3E-07 7.1E-08 61.6 0.011
mutyh 5.0E-05 1.3E-05 1.7E-04 3.6E-05 71.0 0.004
pnkp 8.0E-06 1.6E-06 5.0E-05 1.6E-05 83.8 0.005
U373 ercc1 1.9E-06 8.2E-07 8.4E-06 2.3E-06 77.4 0.018
ercc2 1.2E-07 3.0E-08 2.8E-06 7.9E-07 95.7 0.003
mutyh 2.6E-05 7.6E-06 9.5E-05 1.8E-05 72.9 0.007
pnkp 3.6E-05 9.6E-06 6.5E-05 1.6E-05 44.2 0.013
mgmt 4.2E-06 5.2E-07 2.1E-05 1.0E-06 80.4 0.001

All used siRNAs significantly reduced expression of their respective targets, when compared to the control siRNA (GFP). Measures were performed twice from each cell sample and results were expressed as the mean of at least three independent samples (originating from independent in vitro experiments)