Figure 4. RPS3 knockdown reduced the mitochondrial Ca²⁺ gatekeeper MICU1.

A375 cells were treated with siRNA of RPS3 (50 nM) for 72 hr. The concentration of calcium ions in cells A. and the activities of caspas-3 and -9 B. were determined. The expression of mitochondrial Ca²⁺ gate keeper protein MICU1 C. and its co-localization with MitoTrackerCMXRos D. were examined by Western blot and confocus microscope, respectively.