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. 2015 Aug 17;6(30):29991–30005. doi: 10.18632/oncotarget.5031

Figure 4. PD0325901 and Saracatinib and the combination treatment reduced cell migratory and invasive activity.

Figure 4

Cell lines were treated with DMSO control (Ctrl), or with PD (1 μM), AZ (4 μM) or their combination. A. for cell migration assays, the wounded areas were imaged at 0 h and 36 or 72 h after the monolayer cultures were scratched. Scale bar: 500 μm. B. the gap area of each image was measured, and the wound closure percentages (mean ± SD) were calculated from at least three independent experiments. C. for Boyden chamber Matrigel invasion assays, cells that invaded through to the underside of the transwell filter after 48 h incubation under various drug treated conditions were fixed, crystal violet stained and imaged. Scale bar: 250 μm. D. cell count analysis was performed for each image and the relative cell invasion (mean ± SD) of each treated group compared to the Ctrl group of each cell line was calculated from at least three independent experiments. *,P < 0.05; **,P < 0.01 compared with Ctrl group.