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. 2015 Aug 17;6(30):30006–30016. doi: 10.18632/oncotarget.4933

Figure 3. miR-146b-5p suppresses IL-6.

Figure 3

A. Sequence alignment of human miR-146b-5p binding site in the IL-6 3′UTR, showing also the mutated sequence (bolded italic) B, C, D. and E. Total RNA was extracted from the indicated cells, and the levels of pre-miR-146b-5p, mature miR-146b-5p and IL-6 were assessed by qRT-PCR. Error bars represent means ± S.D. *p value < 0.0009. F. Upper panel, schematic representation of the luciferase reporter vector bearing the miR-146b-5p binding site in the IL-6 3′UTR, Lower panel, cells were stably transfected with the luciferase reporter vector bearing either the wild type IL-6 3′UTR (black bars) or a mutated sequence (shown in a) for the binding site of miR-146b-5p (white bars). The reporter activity was assessed at 48 h post-transfection. Data (Mean ±SEM, n = 3) were presented as % change in reporter activity as compared to the negative control cells. *p value < 0.0078.