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. 2015 Aug 31;6(30):30017–30034. doi: 10.18632/oncotarget.5013

Figure 3. PGAM5L is required for Bax-mediated intrinsic apoptosis.

Figure 3

A–C. SW480, HCT116 WT and HCT116 Bax−/− cells were treated with arenobufagin in the absence or presence of PGAM5L-KD and PGAM5S-KD. The proportion of annexin V-positive cells was analyzed by flow cytometry (n = 3). D. HCT-116 cells or HCT-116 Bax−/− cells were transfected with PGAM5L-KD or PGAM5S-KD. Immunoblot analysis of PARP, Drp1 and Bax were determined after 24 h of arenobufagin treatment. E. Confocal immunofluorescence of Bax (red, anti-Bax) in the PGAM5L-KD and PGAM5S-KD SW480 cells that were loaded with MitoTracker and DAPI. 1000× for all, scale bar = 10 μm. See also Supplementary Figure S3.