A. Effect of Arenobufagin and Cisplatin on the survival ratio of mice. B. Effect of Arenobufagin and Cisplatin on body weight of mice. C. Isolated tumor size and tumor weight from the HCT116 WT and HCT116 Bax−/− mice heterotropic CRC model. D. Microscopic view of colon tumor tissue in mice. E. PARP, Caspase-3, and Bax in tumor tissue lysates from vehicle-, arenobufagin-, and cisplatin-treated mice were detected by western blot analysis. F. HCT116 WT and HCT116 Bax−/− mice were treated with arenobufagin and cisplatin. IP was performed with an anti-PGAM5 antibody. Co-IP Drp1 and Bax was detected by western blotting. *P < 0.05, #P < 0.01, n = 12, one-way ANOVA, post hoc comparisons, Tukey's test. Columns, means; error bars, SEs. See also Supplementary Figure S6.