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. 2015 Aug 13;6(30):30057–30071. doi: 10.18632/oncotarget.4996

Figure 2. Relevance of HER2 in the proliferation of ovarian cancer cells.

Figure 2

Activation A. and total levels B. of EGFR, HER2, HER3 and HER4 in four ovarian cancer cell lines cultured for 12 hours in the presence or absence of FBS. Activated forms of the HER receptors were analyzed by immunoprecipitation with anti-receptor antibodies, followed by anti-PY blot (A). The levels of the receptors were analyzed in total cell lysates with the respective anti-receptor antibodies (B) GAPDH was used as a loading control. C. Knockdown of HER2 in ovarian cell lines. Cells were infected with control vector (pLKO) and viruses including two different short hairpin sequences targeting HER2. Cell extracts were obtained and the receptor expression was measured by immunoprecipitation followed by Western blot with the anti-HER2 antibody. D. HER2 knockdown effect on the proliferation of ovarian cancer cells. Cells were infected with the indicated shRNAs. After selection, cells were then plated and counted after 5 days. Results are plotted as the mean ± s.d. of triplicates with respect to the proliferation of untreated cultures.