Figure 3. DNA damage response after cisplatin treatment.

a. Representative Western blot analysis reporting the expression and phosphorylation of ATM on serine 1981 in cells treated or not with cisplatin. Lamin B was used as loading control. Graphical presentation of p-Atm Ser¹⁹⁸¹ levels from 2 experiments is shown in Supplementary Figure S1c. b. Phosphorylation of H2AX histone (γH2AX, green) detected by immunofluorescence after release from cisplatin treatment. DAPI (blue) was used to counterstain the nuclei. Scale bar: 25 um. c. Representative Western blot analysis visualizing phosphorylation of H2AX after release from cisplatin treatment. Tubulin was used as loading control. Two independent experiments have been performed. d. Representative images for γH2AX foci quantification. e. γH2AX immunolabeled foci from 2–4 slides and 2 independent experiments were scored by automated quantification in 50 nuclei per slide and graphically presented. Mean values and SEM are shown. Statistical analysis results are reported in Supplementary Table S1.