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. 2015 Aug 17;6(30):30165–30177. doi: 10.18632/oncotarget.5030

Figure 4. P65 was regulated by RASSF8 in melanoma cell line.

Figure 4

A. Overexpression of RASSF8 in Wm266-4 cells (Wm266-4-RASSF8) reduced expression of P65 and p-P65 but increased expression of IκBα. B. Knockdown of RASSF8 in M24 cells (M24-RASSF8 shRNA) increased P65 expression. C. NF-κB luciferase was activated by knockdown of RASSF8, and the activity was blocked by super-repressor IκBα in M24 cells. D. Pretreatment of NF-κB inhibitor BAY 11-7082 significantly blocked migration of Wm266-4 which has low RASSF8 expression. E. Pretreatment of NF-κB inhibitor BAY 11-7082 significantly blocked invasion of Wm266-4, which has low RASSF8 expression. F. p-IκBα and p-65 were more inhibited in M24 shRNA than that in M24 Cntl, whereas no change of IκBα was seen when cells were treated by BAY 11-7082. RASSF8 inhibited cell migration and invasion by regulating P65 expression.