Table 2.
Effects of subcutaneous administration of hGHRH(1–29)NH2 analogs on GH release in male rats.
| Peptide | Dose (μg/kg) | Serum GH level at selected time after injection (ng/ml)
|
Relative potency
|
|||||
|---|---|---|---|---|---|---|---|---|
| 0 min | 15 min | 30 min | Compared to GHRH(1–29)
|
Compared to JI-38
|
||||
| 15 min | 30 min | 15 min | 30 min | |||||
| GHRH (1–29) | 100 | 34.5 ± 10.2 | 140.9 ± 24.2 | 65.1 ± 16.5 | 1.00 | 1.00 | ||
| 200 | 74.6 ± 14.1 | 240.0 ± 27.2 | 96.2 ± 3.4 | |||||
| JI-38 | 3 | 33.2 ± 2.8 | 230.0 ± 15.1 | 128.7 ± 13.3 | 63.1 | 111.8 | 1.00 | 1.00 |
| MR-326 | 3 | 43.7 ± 6.4 | 309.2 ± 33.5 | 225.9 ± 19.8 | 92.7 | 303.4 | 1.47 | 2.71 |
| MR-327 | 3 | 31.5 ± 3.4 | 258.7 ± 31.1 | 138 ± 27.9 | 73.5 | 126.5 | 1.16 | 1.16 |
| MR-351 | 3 | 30.6 ± 7.2 | 90.8 ± 20.3 | 65.4 ± 10.1 | 18.8 | 33.6 | 0.30 | 0.30 |
| MR-356 | 3 | 25.3 ± 3.9 | 305.4 ± 40.0 | 152.4 ± 21.8 | 91.2 | 150.9 | 1.45 | 1.35 |
| MR-361 | 3 | 46.1 ± 6.3 | 275.9 ± 46.4 | 142.6 ± 18.4 | 80 | 134.1 | 1.27 | 1.20 |
| MR-367 | 3 | 41.1 ± 5.6 | 244.0 ± 25.1 | 141.2 ± 12.3 | 68.1 | 131.7 | 1.08 | 1.18 |
| MR-401 | 3 | 24.6 ± 5.3 | 217.9 ± 37.0 | 204.1 ± 23.7 | 58.8 | 253.4 | 0.93 | 2.27 |
| MR-403 | 3 | 39.4 ± 5.3 | 608.7 ± 35.3* | 281.7 ± 18.6* | 223.8 | 448.9 | 3.55* | 4.02* |
| MR-404 | 3 | 29.5 ± 3.2 | 320.5 ± 32.7 | 151 ± 28.5 | 97.1 | 148.4 | 1.54 | 1.33 |
| MR-405 | 3 | 21.8 ± 5.9 | 227.9 ± 10.5 | 143.3 ± 6.1 | 62.3 | 135.2 | 0.99 | 1.21 |
| MR-406 | 3 | 21.3 ± 4.8 | 492.6 ± 21.8* | 282 ± 17.8* | 170 | 449.7 | 2.69* | 4.02* |
| MR-407 | 3 | 36.7 ± 10.2 | 248.0 ± 40.5 | 135.2 ± 10.8 | 69.6 | 122 | 1.10 | 1.09 |
| MR-408 | 3 | 37.6 ± 15.5 | 213.3 ± 32.0 | 121.2 ± 8.4 | 57.2 | 100.5 | 0.91 | 0.90 |
| MR-409 | 3 | 18.9 ± 3.1 | 427.4 ± 62.4* | 256.7 ± 20.7* | 141.3 | 380.6 | 2.24* | 3.4* |
| MR-410 | 3 | 34.1 ± 1.2 | 334.0 ± 54.0 | 250.4 ± 28.6* | 102.5 | 363.2 | 1.58 | 3.25* |
| MR-420 | 3 | 35.5 ± 7.6 | 256.2 ± 46.0 | 126.1 ± 15.8 | 72.6 | 107.8 | 1.15 | 0.96 |
| MR-421 | 3 | 44.0 ± 8.9 | 252.4 ± 33.3 | 137.3 ± 17.5 | 71.2 | 125.4 | 1.13 | 1.12 |
| MR-502 | 3 | 61.7 ± 8.1 | 266.7 ± 21 | 153.2 ± 33.3 | 76.5 | 152.3 | 1.21 | 1.36 |
| MR-504 | 3 | 25.4 ± 4.1 | 200.0 ± 10.7 | 130.0 ± 19.1 | 52.6 | 113.8 | 0.83 | 1.02 |
| MR-702 | 3 | 43.7 ± 5 | 103.7 ± 23.1 | 85 ± 27.8 | 22.4 | 53.5 | 0.35 | 0.48 |
Data are shown as mean ± SEM.
*
p < 0.05 vs JI-38 (ANOVA followed by Tukey’s test).