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. Author manuscript; available in PMC: 2016 Feb 8.
Published in final edited form as: Cell Signal. 2014 Jan 18;26(5):1135–1146. doi: 10.1016/j.cellsig.2014.01.009

Fig. 2. Gαq and different Gβγ dimers localized at the mitochondria.

Fig. 2

(A) Confocal micrographs of HeLa cells stably expressing mt-DsRed and Gβ4-Flag γ2-HA and/or Gαq, immunostained with anti-Flag or HA and mounted with DAPI. (B) Mitochondrial fractions of NIH3T3 cells submitted to trypsin digestion in presence or absence of Triton X-100, immunoblotted with anti-Gαq/11, OPA1 (inner membrane), Mfn2 (outer membrane), Porin (integral outer membrane) and Gβ-Pan (representing Gβγ dimer). (C) Diagram showing the likely actions of trypsin on proteins blotted in B. (D) Mouse liver mitochondrial sub-fractions shown the presence of Gαq/11 at the inner membrane, immunoblotted with Gαq/11 and markers: Porin (OM), Smac/Diablo (IMS), COXI (IM) and Hsp70 (M).