Figure 7.
Assay of Ad5-pIX-RFP-FF/NK2 replication.
Notes: The Hep 3B2.1-7 (A), SNU-449 (B), and MCF-7 (C) cells were seeded in triplicates in 24-well plates at 1×105 cells/well. On the following day, the cells were infected with either Ad5-pIX-RFP-FF/NK2 or the Ad5-pIX-RFP-wt/Fiber control at an MOI of 100 VP/cell for up to 4 days. Total DNA was extracted at each time point, and Ad E4 DNA was measured by real-time PCR. Each experimental condition was performed in triplicate, and the experiments were repeated three times. The results were expressed as the mean of the three experiments ± SEM. The Ad5-pIX-RFP-FF/NK2 was compared with the Ad5-pIX-RFP-wt/Fiber virus, using a two-tailed Student’s t-test. The differences were considered statistically significant (*) if P<0.05.
Abbreviations: MOI, multiplicity of infection; VP, viral particles; PCR, polymerase chain reaction; SEM, standard error of the mean; Ad5, adenovirus serotype 5; NK2, a secreted truncated splicing variant that extends through the second kringle domain.