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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2015 Nov 20;11(1):168–181. doi: 10.1007/s11481-015-9643-8

Fig. 6. IL-12B expression is influenced by multiple epigenetic mechanisms and miRNAs.

Fig. 6

Fig. 6

a) IL12 (p70) level in serum of control and PTSD subjects as analyzed by Bioplex assay. For the in vitro studies, THP-1 cells were treated with 5-AZA @1μM concentration in one type of experiment, and transfected with siRNA for KDM5B in another, followed by quantification of IL-12B transcript by real time PCR. b) Transcript level of IL-12B after treatment with 5-AZA for 48 h. c) Transcript level of IL-12B after knockdown of KDM5B. d) Transcript level of KDM5B after it was knocked down by siRNA. e) MiRNA-gene interactive network generated in IPA for the dysregulated miRNAs and some of the select targets. Green indicates down regulated miRNAs and red is for up regulated. The solid arrows indicate direct interaction and broken for indirect. Subsequently, hsa-miR-193a-5p was upregulated in THP-1 cells by transfection with its pre-miR as described in methods to see its effect on IL-12 expression. f) Level of hsa-miR-193a-5p in the PBMCs of PTSD detected by qRT-PCR (control 17 and PTSD 16 subjects). g) Level of IL-12B transcripts in the PBMCs from a healthy control after transfection with pre-miR-193a-5p or its inhibitor. h) IL-12 (p70) detected by ELISA in the culture supernatant after transfection of PBMCs from a healthy control with pre-miR-193a-5p. (RA: relative abundance, RE: relative expression).