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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Methods. 2015 Aug 8;94:51–64. doi: 10.1016/j.ymeth.2015.08.005

Figure 7. Different methods to functionalized PDMS surfaces.

Figure 7

A degassed viscous prepolymer mixture is cast on molds and cured to form arrays of PDMS microposts. PDMS surfaces are functionalized by increasing their surface energy with oxidation using plasma, UV-ozone, piranha, and chemical deposition methods. Once oxidized, proteins can be adsorbed or stamped on surfaces for stable cell adhesion or incubated in organosilanes that contain chemical groups to covalently bind proteins and oxide to bind to the PDMS surface. Laminin is a potential ECM protein that binds to PDMS with this strategy. An alternative approach not involving oxidation consists of incubating PDMS in benzophenone and exposing it to UV to polymerize the acrylic acid on the surface and bind proteins. Figure adapted with permission from [19, 99, 120, 202].