Table 1. Primers and conditions used for PCR amplification of mini- and microsatellite typing, including four novel VNTR regions identified in the C. parvum genome in this study using the SSR Finder software.

Locus	GenBank accession no.	Chromosome	Primer	Primer sequence (5’ → 3’)	Repeat motif	Annealing temp (°C)	Fragment size range (bp)	Reference
ML2	AF344880	VI	F	caatgtaagtttacttatgattat	AG	50	180–237	[19]
			R	FAM-cgactataaagatgagagaag
5B12	AQ449854	II	F	tgacgatgaagatgagggaac	AT	60	134–155	[16]
			R	HEX-caggacagatttaggaggagga
MSB	XM627997	I	F	gggaggcatagggatga	AGATAG	59	246–324	[20]
			R	TAMRA-cttttgatcgcttcttttcca
TP14	XM627041	VIII	F1	taatgcccacccatcttctt	CAA	61	279–333	[16, 21]
			R1	tccatctgggtccatttagc
			F2	ctaacgttcacagccaacagtacc		62
			R2	FAM-gtacagctcctgttcctgttg
ML1	G35348	III	F1	catgagctaaaaatggtgg	GAG	55	218–242	[19, 22]
			F2	ctaaaaatggtggagaatattc		50
			R	HEX-caacaaaatctatatcctc
CP47	AF384127	VI	F1	gcttagattctgatatggatctat	TAA,TGA/TAG	43	417–479	[17]
			R1	agcttactggtcctgtatcagtt
			F2	accccagaaggcggaccaaggtt		55
			R2	FAM-gtatcgtggcgttctgaattatcaa
MSC6-7	BX538350	VI	F1	attgaacaaacgccgcaaatgtaca	TGATGATGAT(G)GAACC(T)	55	517–570	[17]
			R1	cgattatctcaatattggctgttattgc
			F2	gctatttgctatcgtctcacataact		55
			R2	TAMRA-ctactgaatctgatcttgcatcaagt
cgd2_3850	XM626569	II	F1	attgaagattgcggatgatggggtt	CTGCTC(A)	70	151–205	This study
			R1	tggagcgccaagtgctgaaga
			F2	atttgctgttgcaactggtg		61
			R2	TAMRA-gccaagtgctgaagaagagg
cgd1_3670	XM628179	I	F	ggaaaggcattaatcctcca	TGAGCC	60	235–289	This study
			R	FAM-gaactaggctcgggttcaga
cgd6_5400	XM627858	VI	F	taatctttgcgtgggacctc	GTAGTG(A)	60	251–312	This study
			R1	gtgacttgaatgacccagga
			R2	HEX-tggagtttctgagacacaaaga		59
cgd6_3940	XM627731	VI	F	agtggtcttggagcctgaga	TTGGCA	62	312–342	This study
			R	TAMRA-acttggacaagtgccaggtc