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. 2016 Feb 9;7:110. doi: 10.3389/fmicb.2016.00110

FIGURE 5.

FIGURE 5

Growth properties of 562 derivative viruses carrying various adaptive mutations in gp120. (A) Growth kinetics of variant viruses carrying a single adaptive mutation. Virus samples were prepared from 293T cells transfected with indicated proviral clones, and inoculated into HSC-F cells. Virus replication was monitored by RT activity released into the culture supernatants. Data of left and right panels were obtained from the same experiment, and the same result for 562 is separately shown as a control. Infection condition: 2 × 105 RT units/2 × 105 cells. (B) Growth properties of slowly growing variant viruses carrying a single adaptive mutation. Experiment was performed as described above. Infection condition: 4 × 106 RT units/106 cells. (C) Growth kinetics of variant viruses carrying two adaptive mutations. Experiments were performed as above. LF, L124F; NK, N132K; GR, G150R; FL, F174L; SG, S304G; IV, I307V; GR, G310R. Infection conditions: left, 2 × 105 RT units/2 × 105 cells; right, 5 × 104 RT units/1 × 105 cells.