FIGURE 8.

Effects of amino acid substitutions on the stability and affinity of gp120-CD4 complex. Full-length 562 gp120-CD4 complex model was constructed as described in section “Materials and Methods,” and used for in silico mutagenesis (Nomaguchi et al., 2013b). Changes in the stability (A) and affinity (B) scores by single amino acid substitutions were computed by using the Protein Design application in MOE as described in section “Materials and Methods.” Bars indicate standard deviations of the score (n = 3).