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Representative 3,3-diaminobenzidine immunohistochemical (A,B) and in situ hybridization (C,D) stained sections of proliferating (A,C) and involuted (B,D) infantile hemangiomas (IH) demonstrating the absence of AFP [(A,B), brown; (C,D), red]. The IHC-stained IH sections were also stained with SMA [(A,B), red], identifying the pericyte layer of the microvessels. All slides were counterstained with hematoxylin to identify cell nuclei [(A–D), blue]. Image magnification: 400×.
