Figure 5.

ALA promotes the pollen Ca²⁺-ATPase activity. Pollens were germinated in simplified culture, containing 10% sucrose and 0.01% H₃BO₃, or with 5–20 mg/L ALA, and incubated at 25°C at 100% humidity, in the dark for 3 h. Then Ca²⁺-ATPase activity was assayed using the Ca²⁺-ATPase assay kit. Values are the means of 9 measurements ± SE from three independent experiments. Different small letters represent significant difference between treatments (P < 0.05).