Figure 11.

Expression and localization of redox-sensitive TRP channel in the human liver tissue. (A) Expression of mRNAs for TRPV1-4, TRPC1, TRPC4, TRPC5, TRPM2, TRPM7, TRPA1, and GAPDH detected by PCR in the cDNA library from normal human liver. Specific PCR primers used are listed in the Table 1. (B) In situ hybridization analysis of TRPV1, TRPC1, TRPM2, TRPM7, and TRPA1 mRNAs in the paraffin section of the human liver. mRNAs of TRPV1 (a,f), TRPC1 (b,g), TRPM2 (c,h), TRPM7 (d,i) were localized in hepatocytes (^*) and Kupffer cells (arrow). TRPA1 (e,j) was localized in the lining of sinusoids and Kupffer cells of the human liver tissue. The images from the analysis of both the antisense (left) and sense probes (right) are shown. Scale bar, 100 μm.