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. 2016 Feb 9;7:19. doi: 10.3389/fphar.2016.00019

Figure 3.

Figure 3

Expression of redox-sensitive TRP channels in HepG2 cells and their responses when expressed in HEK293 cells. (A) Expression of redox-sensitive TRP channel mRNAs [TRPV1-4, TRPC1, TRPC4, TRPC5, TRPM2, TRPM7, TRPA1, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)] detected by RT-PCR in total RNA isolated from HepG2 cells. Specific PCR primers used are listed in Table 1. (B,C) [Ca2+]i responsesevoked by 20 mM APAP (B) or 1 mM H2O2 (C) in HEK293 cells expressing human TRPV1, human TRPV2, human TRPV3, human TRPV4, mouse TRPC1, mouse TRPC4β, mouse TRPC5, human TRPM2, human TRPM7, human TRPA1, or vector. Average time courses (left) and Δ[Ca2+]i (right) (n = 16–64). Data points are mean ± SEM. P≥0.05, *P < 0.05, **P < 0.01, and ***P < 0.001 compared to vector. Differences not statistically significant are labeled as (ns). All data were analyzed by Student's t-test.