Figure 4.

Inhibition of APAP- or H₂O₂-induced Ca²⁺ entry and ROS production by selective TRP channel blockers in HepG2 cells. (A) Effects of selective blockers capsazepine (CPZ; 10 μM) for TRPV1, 2-APB (100 μM) for TRPC1, clotrimazole (CTZ; 50 μM) for TRPM2, or AA861 (10 μM) for TRPM7 on [Ca²⁺]_i rises evoked by APAP (20 mM). Average time courses (left) and Δ[Ca²⁺]_i (right) (n = 18–57). (B) Effects of 10 μM CPZ, 100 μM 2-APB, 50 μM CTZ, and 10 μM AA861 on [Ca²⁺]_i rises evoked by H₂O₂ (1 mM). Average time courses (left) and Δ[Ca²⁺]_i (right) (n = 19–43). (C,D) Effects of selective blockers of TRP channels on ROS production evoked by APAP (20 mM) (C) or H₂O₂ (1 mM) (D). Blockers are applied for 3 h before and during APAP or H₂O₂ stimulation. Data points are mean ± SEM. ^***P < 0.001 compared to DMSO. All data of [Ca²⁺]_i measurements were analyzed by Student's t-test, while those of ROS measurements were analyzed by ANOVA and Bonferroni post-hoc.