Figure 8.

Effects of APAP on the activity of caspase 3/7 and the level of intracellular cytochrome c in HepG2 cell. (A,B) ROS scavengers, NAC or tiron, (1 mM) suppressed APAP- or H₂O₂-induced increases of the activity of caspase 3/7 (A) and the level of intracellular cytochrome c (B) in HepG2 cells. (C,D) Selective Ca²⁺ channels blockers, 1 μM of either CPZ, 2-APB, CTZ, and AA861 suppressed APAP- or H₂O₂-induced increases of the activity of caspase 3/7 (C) and the level of intracellular cytochrome c (D) of HepG2 cells. (E,F) siRNA-mediated knockdown of TRPV1, TRPC1, TRPM2, and TRPM7 suppressed APAP- or H₂O₂-induced increases of the activity of caspase 3/7 (E) and the level of intracellular cytochrome c (F) in HepG2 cells. Data points are mean ± SEM. P≥0.05, ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001 compared to the DMSO, siScramble or control. Differences not statistically significant are labeled as (ns). All data were analyzed by ANOVA and Bonferroni post-hoc.