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The effects of APAP on the covalent incorporation of DTNB-2Bio in TRPV1, TRPC1, and TRPV4 proteins. (A) Chemical structure of DTNB-2Bio (Yoshida et al., 2006). (B–D) Western blot using an anti-GFP or anti-Flag antibody of avidin-bound fractions (top panel) and total cell lysates (bottom panel) prepared from HEK293T cells which were transfected with EGFP-TRPV1 (B), TRPC1-Flag (C), EGFP-TRPV4 (D), and then incubated with DTNB-2Bio in the presence or absence of 20 mM APAP.
