Skip to main content
. 2016 Feb 9;7:99. doi: 10.3389/fpls.2016.00099

FIGURE 4.

FIGURE 4

Screening for homozygous DnAGL19 overexpressing Arabidopsis thaliana lines. (A) Detection of the DnAGL19 insertion in putative transgenic lines. Genomic DNA was extracted from wild type A. thaliana and putative transgenic lines marked #1 to #15. The DnAGL19 sequence (639 bp) was amplified using genomic DNA as a template. (B) Growth of identified homozygous plants on a MS plate containing 50 mg/L kanamycin. Lines # 1, # 3, # 4, # 9 and wild type are shown. (C) Expression of DnAGL19 in transgenic lines. Total RNA was extracted from lines # 1, # 3, # 4, # 9 and wild type seedlings and reversed transcribed. RT-PCR was performed to detect the expression level of DnAGL19.