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. 2016 Feb 9;7:99. doi: 10.3389/fpls.2016.00099

FIGURE 5.

FIGURE 5

Phenotypes of Arabidopsis thaliana lines overexpressing DnAGL19. (A) Growth of 25-day old wild type (WT) and DnAGL19 overexpressing lines (35S::DnAGL19-6myc #9 and #4) under normal LD condition. (B) Expression of LFY and AP1 at different developmental stages in wild type and DnAGL19 overexpressing lines (#1 and #4). Total RNA was extracted from seedlings of wild type and the overexpressing lines growing under LD conditions. RT-PCR was carried out. The developmental stages are shown as days after sowing. (C) Expression of flowering time associated genes (HOS1, FT, FLC, and SOC1) in wild type and DnAGL19 overexpressing seedlings before and after vernalization. “N” indicates the non-vernalized plants and “V” indicates the vernalized ones. Total RNA was extracted from 10-day old seedlings. Real-time qPCR was carried out in triplicates for each sample/gene combination, and data are shown as mean ± SD, with ∗p < 0.05 and ∗∗p < 0.01.