Skip to main content
. 2016 Jan 17;17(4):701–710. doi: 10.22074/cellj.2016.3842

Table 1.

Primers used for reverse transcription polymerase chain reaction (RT-PCR) analysis


Genes Primer sequences

Pdx1 F: 5´CCACCCCAGTTTACAAGCTC3´
R: 5´TGTAGGCAGTACGGGTCCTC3´
Ngn3 F: 5´CTGCGCATAGCGGACCACAGCTTC3´
R: 5´CTTCACAAGAAGTCTGAGAACACCAG3´
glucagon F: 5´ACCAAGCCGTCATCTTCCAG3´
R: 5´CGTATAGGGCACGTAGCAGG3´
Amylase F: 5´CATTGTTGCACCTTGTCACC3´
R: 5´TTCTGCTGCTTTCCCTCATT3´
Insulin1 F: 5´TAGTGACCAGCTATAATCAGAG3´
R: 5´ACGCCAAGGTCTGAAGGTCC3´
Insulin2 F: 5´CCCTGCTGGCCCTGCTCTT3´
R: 5´AGGTCTGAAGGTCACCTGCT3´
β-actin F: 5`ATGAAGATCCTGACCGAGCG3`
R: 5`TACTTGCGCTCAGGAGGAGC3`