Fig. 7.

T-lymphocytes resist shear detachment through the β₂ integrin. (A) T-lymphocytes pretreated with blocking antibodies against the β₁ (red bar) or β₂ (green bar) integrin subunits and were exposed to ICAM-1, VCAM-1, or ICAM-1 + VCAM-1 surfaces under 100 s⁻¹ of fluid flow and the MI was determined. Data are mean ± SE (B) T-lymphocytes were exposed to increasing shear rates and the percent of cells remaining bound after 5 minutes of exposure was calculated. Shear rate was increased every 5 minutes starting at 100 s⁻¹ and ending at 2000 s⁻¹.